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Please use this identifier to cite or link to this item: http://hdl.handle.net/123456789/227

Title: Screening of the antimutagenic activity mushroom (pleurotus ostreatus) using the mouse bone marrow micronucleus test
Authors: Mangallay, Anna Liza B.
Keywords: Screening
Antimutagenic activity
Oyster mushroom (pleurotus ostreatus)
Bone marrow,
Micronucleus test
Issue Date: Nov-2004
Abstract: THESIS ABSTRACT Title: SCREENING OF THE ANTIMUTAGENIC ACTIVITY of the OYSTER MUSHROOM (Pleurotus ostreatus) USING THE MOUSE BONE MARROW MICRONUCLEUS TEST Total No. of Pages: 114 Text No. of Pages: 90 Author: ANNA LIZA B. MANGALLAY Type of Document: Thesis Type of Publication: Unpublished Accrediting Institution: Saint Louis University, Baguio City Keywords: Screening, Antimutagenic Activity, Oyster Mushroom (Pleurotus ostreatus), Bone Marrow, Micronucleus test Summary This study was conducted to determine the physiologically active compounds present in the oyster mushroom extract (Pleurotus ostreatus) and to screen the antimutagenic activity of the different concentrations of the oyster mushroom extract (Pleurotus ostreatus) to 4-Nitroquinoline-1-oxide in the bone marrow cells of mice. Specifically, it aimed to find out the answers to the following questions: 1. What are the physiologically active compounds present in the oyster mushroom (Pleurotus ostreatus) extract? 2. Which concentration (25%, 50% and 75%) of the oyster mushroom extract (Pleurotus ostreatus) is able to decrease the number of Micronucleated Polychromatic Erythrocytes (MPCEs) by at least one-half that of the control that received the 4-Nitroquinoline 1-Oxide only in the bone marrow cells of mice? 3. Is there a significant difference in the number of Micronucleated Polychromatic Erythrocytes (MPCEs) in the bone marrow cells of mice that received the different concentrations (25%, 50% and 75%) of the oyster mushroom extract (Pleurotus ostreatus)? The study was conducted at Pinsao Pilot Project and at the Natural Sciences Research Unit, Saint Louis University, Baguio City from June to October 2004. The study made use of the experimental method of research utilizing Mouse Bone Marrow Micronucleus Test introduced by Schmid to detect antimutagenic activity of the oyster mushroom (Pleurotus ostreatus) extract to 4-Nitroquinoline-1- oxide in the bone marrow cells of mice. The data gathered were statistically validated using the Analysis of Variance (ANOVA). The differences in the means were analyzed using the Tukey HSD Test. Findings Based on the analysis and interpretation of data obtained, the researcher presents the following findings: 1. Oyster mushroom (Pleurotus ostreatus) contains different physiologically active compounds such as the alkaloids, flavonoids, and terpenoids. 2. All the concentrations (25%, 50% and 75%) of the oyster mushroom (Pleurotus ostreatus) decrease the number of Micronucleated Polychromatic Erythrocytes by at least one-half in the bone marrow cells of mice treated with 4-Nitroquinoline-1-Oxide. 3. There is no significant difference in the number of Micronucleated Polychromatic Erythrocytes (MPCE) formed in the bone marrow cells of mice treated with the different concentrations of the oyster mushroom extract (Pleurotus ostreatus), 25%, 50% and 75%. Conclusion From the foregoing findings, the following conclusion were drawn: 1. The oyster mushroom (Pleurotus ostreatus) contains physiologically active compounds to which the antimutagenic activity may be attributed. Among these are the alkaloids, flavonoid and terpenes. 2. All the concentrations of the oyster mushroom extract (Pleurotus ostreatus): 25%, 50%, 75% elicited antimutagenic activity of 4-Nitroquinoline-1-Oxide. 3. Concentration has no effect on the degree of antimutagenic activity elicited by the oyster mushroom extract (Pleurotus ostreatus) against 4-Nitroquinoline- 1-Oxide. Recommendations From the conclusion derived from the study, the following are recommended: 1. Screening of other species of mushroom in Baguio City and the Cordillera Region, aside from oyster mushroom (Pleurotus ostreatus). 2. Performance of other mutagenic assays to further verify and confirm the results. 3. Performance of pre-clinical efficacy studies to fully evaluate the mechanism of action of oyster mushroom (Pleurotus ostreatus) as antimutagenic substance. 4. Isolation, purification and structural elucidation of the physiologically active compounds responsible for eliciting the antimutagenic activity. 5. Formations of suitable dosage form for oyster mushroom (Pleurotus ostreatus) as antimutagenic substance.
Description: Thesis(M.S.:Biology)--Saint Louis University,BaguioCity,2004.
URI: http://hdl.handle.net/123456789/227
Appears in Collections:School of Natural Sciences

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