Callous tissue formation on selected monocot and dicot plants using coconut milk and coconut water

Abstract

THESIS ABSTRACT Title: CALLOUS TISSUE FORMATION ON SELECTED MONOCOT AND DICOT PLANTS USING COCONUT MILK AND COCONUT WATER Total No. of Pages: 126 Text No. of Pages: 96 Author: FILIPINAS C. DUNGCA Type of Document: Thesis Type of Publication: Unpublished Host/Accrediting Institution: Saint Louis University (Private) Bonifacio St., Baguio City CHED Key Words: Callous Tissue Formation, Selected Monocot, Dicot Plants, Coconut Milk, Coconut Water Abstract: Summary The study was conducted to determine the extent of callous tissue formation on selected monocot and dicot plants which were treated with different concentrations of coconut milk and coconut water with GA as control. The effects of the different concentrations were determined in terms of the number of callous tissues formed on the explants within a period of two weeks. Statistical tools employed were two way analysis of variance in the form of F-test and t-test. Findings The following results were obtained during the conduct of the study: Of the two monocot explants used in the study, only the corn explants developed callous tissues in the following order of abundance: seeds, roots, stems. Coconut water had induced the most extensive callous tissues on the corn explants. Concentrations 1:300 and 1:400 of coconut water had induced more callous tissues among the seed explants while for the stem explants concentrations of 1:300 of coconut milk had stimulated more callous tissues formation. Among the dicot plants used in the study, the tomato explants had the most number of callous tissues formed, specifically the seed explants followed by the roots and lastly by the stem. No growth was observed on the leaf explants. Concentration 1:400 of coconut milk had accelerated more callous tissues on the stem explants followed by 1:400 solution of coconut water. Concentrations 1:200 of coconut milk and 1:300 of coconut water had stimulated more callous tissues formation on the root explants. There was acceleration of callous tissues formation on the seed explants in concentrations 1:100 and 1:200 of coconut water. Conclusion The media used in the study on the monocot and dicot explants showed that coconut water stimulated more callous tissue formation as manifested on the seeds, roots and stems. Concentrations 1:100, 1:200, 1:300 accelerated callous tissues formation on the seed explants and 1:200, 1:300, 1:400 solutions induced calli to form on the root explants. Stimulation of callous tissue formation on the stem explants occurred in concentration 1:400. Recommendations The use of coconut milk and coconut water is recommended for induction of callous tissues formations specially in conducting experiments and researches on plant propagation and also in backyard gardening. Better results of the study can be achieved if the explants are obtained from the meristematic regions.