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Please use this identifier to cite or link to this item: http://hdl.handle.net/123456789/333

Title: Staphylococcal Lysate Test: an alternative for one-stage prothrombin time determination
Authors: Laranang, Wilson Leal
Keywords: Staphylocoagulase
prothrombin time
percent activity
Issue Date: Mar-2010
Abstract: The study was conducted to assess the effectiveness of the combined Staphylococcal whole cell lysate — crude partial coagulase filtrate suspension (SLT study reagent) as an alternative reagent for one-stage prothrombin time (PT) assay as compared to the commercial lyophilized rabbit brain tissue thromboplastin (Hemoplastin) using various concentrations of fresh normal plasma pool as determined by the agreement in PT and its resultant percent activity (%A). Automated coagulometer using optical detection was used with a reference setting limited to 100 seconds having a manually computed %A of 5.8. Correspondingly, the values of the PT and its %A were arbitrarily assumed at 12.5% and 10% with Hemoplastin and 25%, 12.5% and 10% with the SLT study reagent. Based on Bland and Altman analysis, statistically inconsistent agreement was noted in PT intercept 0.0047, p < 0.0125; slope 0.0127, p > 0.0125 and the %A intercept 0.9914; slope 0.0468 both p > 0.0125, respectively. The above findings indicate that the SLT study reagent is not effective as an alternative reagent as compared to Hemoplastin. Further fractionation, isolation, purification and standardization of the study reagent, mean PT and ISI calibration using factor deficient plasma controls and use of other kinetic coagulation detection methods were suggested
Description: Thesis (M.S. : Medical Technology). -- Saint louis University, Baguio City, 2010.
URI: http://hdl.handle.net/123456789/333
Appears in Collections:School of Natural Sciences

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